: Utilizing The computer software in your qPCR thermocycler, determine at which cycle the fluorescence depth crosses a user‐specified threshold. Employing optimistic and negative controls as benchmarks, go with a threshold that clearly distinguishes amplification from non‐amplification. If a solution of proteinase K (PK) is used for sample collection (Simple https://www.facebook.com/NewTechFind/posts/pfbid02L5oyTQPktFpQgGm7RXoC6AG2aMk2JMSeACVRvwrC57B6EG7BVkq8kv2jvWFUJUuYl